Abstract
Instrumental thin-layer chromatography (TLC) offers automation, reproducibility, and accurate quantification for a wide variety of applications. This chapter is focused on an overview of the instrumental TLC applied to the dye and ink analysis. Even though TLC is a destructive chromatographic technique, it is widely used to separate and identify compounds from different matrices due to its capability to generate qualitative information unavailable through the nondestructive spectroscopic techniques. Instrumental TLC occupies a very important place in the advances obtained in the separation, identification, and quantification of dyes from various matrices such as food, textiles, cosmetics, inks, and so on. The performances of different instrumental TLC methods are highlighted by the use of dyes as test compounds. Instrumental TLC is generally accepted as a scientific methodology used to compare and characterize dyes from different ink formulations having the highest discrimination power for the individual techniques.
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