Abstract
This chapter explains the solid-phase extraction on molecularly imprinted polymers (MIPs) and reviews several examples of highly selective pre-concentration of biologic and environment samples. A generic understanding of imprint-analyte binding mechanisms and the various physicochemical binding modes to these extraction materials is essential for a successful development of selective molecular-imprint based solid-phase extraction (MISPE) methods.. Compared with immunoaffinity materials, MIPs have the clear advantage of being highly stable under a large range of buffer pH, solvent, temperature, and pressure conditions, thereby allowing large opportunities for selection of the best experimental conditions for the most efficient use of MIPs. An interesting example is postpolymerization hydrophilization of the external surface of monodisperse MIP beads to render them more compatible with proteins in biosamples, thus allowing direct injection of serum samples into a highly selective MIP pre-column in a coupled-column system. In addition, MISPE with direct detection of the analyte elution is appealing as it eliminates the need for an analytic column, which would simplify the overall separation system and increase the speed of analysis.
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