Abstract
Publisher Summary This chapter discusses the isolation and characterization of DNA polymerases from eukaryotic cells and briefly describes these DNA polymerases, providing a methodology by which procedures for assaying and isolating DNA polymerases from different tissues may be worked out. More detailed information about the specific DNA polymerases identified in different eukaryotic cells can be obtained from several recent reviews. It is not clear how many DNA polymerases are present in eukaryotic cells or whether different tissues have different polymerases. The role of each of these enzymes in either DNA replication or repair has not been established unequivocally. An understanding of the relationships among the different polymerases, their mechanism for catalysis, and their cellular function awaits future experimentation. Extensive purification is required as a prerequisite for answering many such questions.
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