Abstract

After its first industrial uses, dating back to the 1950s, Yarrowia lipolytica has aroused interest as host for heterologous protein production. This oleaginous yeast has become a recognized expression system, with a remarkable secretion capacity. The development of tools for engineering its metabolic pathways has allowed its use as cell factory in a variety of bioconversion processes, notably single-cell oil and biofuel production and also valorization of industrial wastes into high-value compounds. The more recent development of new DNA assembly methods and of CRISPR genome editing technologies has boosted further the possibilities for Y. lipolytica engineering. Consequently the ever-increasing number of publications makes it now difficult for new players to acquire a general overview on the field. This chapter aims to provide basic knowledge on the subject, to direct novice readers to most useful recent reviews, and to provide an update on recently developed engineering tools and technological applications.

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