Abstract
This chapter discusses the induction of mammalian somatic cell hybridization by polyethylene glycol (PEG). The hybridization of somatic cells is a technique for the study of the organization and regulation of the mammalian genome. PEG induces mammalian cell hybridization and procedures have been developed for treating cells with PEG that are simple, rapid, and effective. The chapter outlines experiments to optimize the conditions of PEG-induced hybridization of cells attached in monolayers. Among the parameters considered were: (1) PEG molecular weight, (2) PEG concentration, (3) cell density, (4) pH, (5) length of exposure to PEG, (6) temperature, (7) volume of residual medium at the time of PEG addition, and (8) the method of removing the PEG. The experience with PEG-induced hybridization of cells in suspension is less extensive than with cells in monolayer; however a simple procedure giving consistent success with certain cell combinations has been developed. Although only a few variables have been examined for hybridization in suspension, it seems apparent that the principles established with monolayer fusions cannot be extrapolated to suspension fusions without some modification.
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