Abstract
This chapter discusses the methodology of bone histomorphometry and highlights the tissue-level characteristics of normal and abnormal bone development. Bone histomorphometry has been a key tool for studying bone metabolism and, to a lesser extent, bone mass and structure. Histomorphometry of undecalcified bone samples is a method to directly obtain quantitative information on bone. It helps determine the amount and distribution of bone tissue with unsurpassed resolution. When tetracycline labeling is performed prior to biopsy, bone histomorphometry offers the unique possibility to study bone cell function in vivo. At the same time, qualitative histologic assessment is possible, which allows the detection of subtle disorders of bone structure. The growth process does not interfere with the measurements. Bone histomorphometry is also an excellent educational tool; the insight gleaned from studying bone tissue can be used to better understand results of indirect methods of bone analysis.
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