Abstract
To study the functions of a peptide family, one needs to localize individual members of the peptide family, as well as the various subtypes of receptors. This chapter describes the use of antibodies that distinguish between luteinizing hormone-releasing hormones (LHRH-I) and LHRH-II to delineate the distribution of the different LHRH-like peptides in the frog nervous systems. Sympathetic ganglia have provided a good system for this study because of their simple anatomy. Neurons in these ganglia are unipolar and have no dendrites. Preganglionic fibers make synaptic contacts almost exclusively on the ganglionic cell bodies. This arrangement facilitates the analysis of the physiological effects of neurotransmitters and allows an accurate anatomical description of synaptic connections. LHRH-I is apparently identical to mammalian pituitary LHRH and seems to be the predominant form in adult frog brain. The LHRH-like transmitter in frog sympathetic ganglia is immunologically similar but not identical to mammalian LHRH. Based on immunological and chromatographical criteria, it has been proposed that LHRH-II is identical to teleost hypothalamic LHRH, which differs from mammalian hypothalamic LHRH by two amino acids at positions 7 and 8.
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