Abstract
This chapter highlights that ergothioneine is a promising, safe, and effective antioxidant for controlling lipid oxidation–induced changes in food quality. It can also be useful for preventing various oxidative diseases associated with stress. Given the importance of this compound, fast, sensitive, and accurate quantitative determination is essential to understand its existence and bioavailability in foods and biological materials. Since the discovery of ergothioneine, numerous methods have been devised for its detection and quantification in biological samples. To evaluate the antioxidant activity of pure ergothioneine and ergothioneine-containing extracts, several methods have been used. The growing interest in investigating ergothioneine's presence and role as an antioxidant in novel resources has raised the demand for the antioxidant's rapid screening in complex mixtures. An online HPLC–DPPH method has been widely applied for rapid screening antioxidant compounds in a large number of extracts. The method is based on a post-column reaction of the HPLC separated antioxidants with the DPPH radical. Consequently, it provides a good analytical tool for screening ergothioneine in biological samples. The chapter provides an overview of the powerful antioxidant ergothioneine and its potential application to prevent lipid oxidation in foods and biological systems. It discusses the developmental history of the methods used for its analysis in biological materials, its bioavailability in humans and animals, and its role in preventing oxidative stress. It also reviews the online HPLC–DPPH method for the evaluation of radical-scavenging compounds in complex mixtures and its application for detecting ergothioneine in mushroom samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
