Abstract
The primary source of world's energy supply has been fulfilled by the fuels as crude oil, coal, and natural gas, yet the energy demand has been continuously increasing. Advanced biofuel except 1-butanol, 2-propanol, and bioethanol has been generally produced only in trace amount. The biofuel produced from the plant biomass as a substrate has been used for the enzyme activity, reaction chemistry, and in the genetics. However, advanced biofuel can also be produced with the help of microorganism through the process of synthetic biology. Through this way, simple sugars have been used for the production of advanced renewable biofuel. Metabolic engineering has been used in the synthetic biology to produce various types of biofuel with the help of microbial hosts including heterotrophic, photoautotrophic bacteria, and yeast.
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