Abstract
Publisher Summary Many tissues in the adult mammalian body continue to have cell proliferation throughout the life of the animal. This chapter discusses method that can be used for labeling every cell that comes into DNA synthesis in tissue–cell populations of an intact mammal (mouse). The method can be used to study slowly renewing cell populations that have not previously lent themselves to quantitative analysis of cell renewal. Specifically, this method involves administration of thymidine-H 3 in the drinking water, followed by tissue autoradiography. This method does not disturb normal cell proliferation of the animal. The dosages used in this method show no detectable radiation toxicity. Some future applications of the method are mentioned. Awareness that such a cell renewal process occurs in the body of adult vertebrates goes back to the past century when it was realized that mitotic activity in the basal layer of epidermis produces new cells at a rate faster than could be accounted for on the bases of growth of the epidermis alone. C. P. Leblond and several of his associates have confirmed and extended the concept of renewal cell populations.
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