Chapter 10 - Isolation and structure elucidation of bioactive polyphenols

Abstract

Abstract Recent development in analytical methods provides significant information necessary for bioactive natural products isolation and structure elucidation. Chromatographic separation, mass and NMR spectroscopic identification tools represent a highly dynamic field with massive recent advances. Bioactive polyphenols are attractive phytochemical group with wide range of diverse effects as well as various biological activities. In this review, the use of recent analytical methods for chemical characterization, isolation and structure elucidation are discussed, organized by analytical techniques and phytochemical class, including simple polyphenolic acids, flavonoids, tannins, xanthines, lignans, coumarins and other related compounds published between 2012 and 2017. A table is structured to summarize all techniques used in publications devoted for bioactive polyphenols separation, isolation and structure elucidation. The recent separation and isolation techniques including column chromatography, HPLC, GC, and TLC are discussed. Additionally, the advances in spectroscopic techniques such as 1D and 2D NMR, UV, MS, LC-MS, GC-MS are reviewed. Moreover, the role LC-MS plays throughout the discovery of new phenolic compounds, where applications range from simple characterization to full structure identification is deliberated.