Abstract
This chapter describes the human arterial wall cells and tissues in culture. Human arterial wall explants and isolated smooth muscle cells (SMCs) have been examined by methods of cell culture for several purposes such as morphological phenotypic stability and replicative characteristics and replicative life span. Arterial wall SMCs derived from the media have been cultivated from tissues obtained at surgery or postmortem. Tissue fragments 1–2 mm in diameter are generally used in this procedure. To obtain these fragments, tissue is aseptically removed from the desired organ and placed on a sterile petri plate. The tissue is minced into smaller and smaller pieces with the use of scalpel blades until the desired size is obtained. Human SMCs grow in the same types of media and at the same serum concentrations that have been used successfully for human and animal fibroblasts. SMCs from human fetal aortas undergo senescent changes and cease dividing after 20 passage generations.
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