Abstract
This chapter discusses the basic principles of a method of nucleoli isolation. The isolation of nucleoli requires, on the one hand, a differential treatment by means of physical or enzymatic agents which do not disrupt the nucleoli but disperse or destroy extranuclear chromatin, and on the other hand, the separation of the nucleoli from the nuclear residue. The Nucleolus contains an excellent technical review of the subject. It has frequently been found that good results are obtained from these techniques only by applying them to the biological material for which they were specially developed. Empirically, modification of these techniques tends to be difficult and unsatisfactory with other material. It is well known that different factors affect structural components of isolated nuclei.
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