Abstract
Publisher Summary Procedures for the isolation of nuclei are important in studies on histones and other nuclear proteins, nuclear enzymes, and nuclear RNA and DNA. The objectives of procedures for isolation of nuclei are to secure a product where (1) the nuclei are morphologically identical to those of the whole cell, (2) the contents of the nuclei as they exist in the cell are all present in the isolated product, and (3) the isolated nuclei do not contain cytoplasmic constituents. The basis for the compression-decompression procedure for isolation of nucleoli is that such delicate structures as chromosomes can be obtained from nuclei without serious damage to their morphology by alternate aspiration and ejection of nuclear suspensions from a syringe. Whereas, the basis of the chemical technique for preparation of the nucleolar fraction is successive extraction of the nuclei with dilute and concentrated saline solution. The chapter also outlines the sucrose–calcium procedure for isolation of nuclei, the citric acid procedure for isolation of nuclei, the use of detergents for nuclear isolation, and the sonication procedure for isolation of nucleoli.
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