Abstract
Publisher Summary This chapter is concerned with techniques applied to mammalian development. Some of these procedures may become as widespread as paraffin embedding in future developmental biology laboratories. More than any other branch of developmental biology, mammalian embryology fits into the society where the work proceeds. Its progress is swayed and succored by society's demands for practical applications. The field responds with programs of research that would be quite foreign to those investigating the development of nematodes and fruit flies. Mammalian developmental techniques are now in the marketplace. Jaenisch and Mintz achieved somatic integration of simian virus 40 (SV40) DNA sequences after injection into the blastocyst; similar results were obtained after injection into pronuclei at the one-cell stage and by 1986 these apparently subtle procedures are “made available to everyone,” at a price. The technique has also started to appear in regular gene-handling manuals.
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