Abstract
Ruminant animals use their symbiotic microorganisms in the rumen to hydrolyze plant fibers and generate energy and other nutrients, while the eukaryotic community (fungi) makes up about half of the total microbial biomass and plays a critical role in the effectiveness of use of lignocellulosic particles of feed components. The aim of the study was to develop a way to increase the efficiency of feed use by ruminants through the introduction of a composition of promising substances into the diet. The assessment of biodiversity included the following: sampling, outflow, purification, measurement of DNA concentrations, PCR, validation and normalization of libraries, followed by sequencing on the platform of high-performance sequencer MiSeq Illumina (USA). Introduction of new substances in the diet of cattle contributes to a change in the eukaryotic composition of the rumen microflora towards an increase in Ascomycota and Neocallimastigomycota phylums (when used together with Quercus cortex extract), which have cellulose and ligninolytic properties, more active development of Chytridiomycota (separate use of substances).
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