Year-end Sale % OFF 
Abstract
Chromosomal inversions are recurrent rearrangements that occur between different plant isolates or cultivars. Such inversions may underlie reproductive isolation in evolution and represent a major obstacle for classical breeding as no crossovers can be observed between inverted sequences on homologous chromosomes. The heterochromatic knob (hk4S) on chromosome 4 is the most well-known inversion of Arabidopsis. If a knob carrying accession such as Col-0 is crossed with a knob-less accession such as Ler-1, crossovers cannot be recovered within the inverted region. Our work shows that by egg-cell specific expression of the Cas9 nuclease from Staphylococcus aureus, a targeted reversal of the 1.1 Mb long hk4S-inversion can be achieved. By crossing Col-0 harbouring the rearranged chromosome 4 with Ler-1, meiotic crossovers can be restored into a region with previously no detectable genetic exchange. The strategy of somatic chromosome engineering for breaking genetic linkage has huge potential for application in plant breeding.
Highlights
Chromosomal inversions are recurrent rearrangements that occur between different plant isolates or cultivars
Besides simple mutations leading to alterations of one or more bases, changes of chromosome structure can be repeatedly observed during the evolution of different species[1,2,3,4]
It was shown for plants that quantitative trait loci (QTLs) can be located in inverted regions[9,10,11], which can cause serious problems if certain genes should be transferred between different cultivars by crossing[12,13]
Summary
Chromosomal inversions are recurrent rearrangements that occur between different plant isolates or cultivars. Five out of ten positive tested T2 lines were analyzed in detail and each of these lines showed at least one positive plant carrying the reverted knob (rknob) inversion. The detection of a chromosome inversion within the respective pools was carried out with the help of junction-specific primer combinations (Fig. 1b).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
