Abstract
To explore the role of ubiquitin-proteasome system (UPS) in the injury of human umbilical vein endothelial cells (HUVECs) induced by oxidative low density lipoprotein (ox-LDL). After exposure to ox-LDL, the levels of ubiquitin (Ub), E1 (ubiquitin activation enzyme), E2 (ubiquitin conjugating enzymes) and E3 (ubiquitin protein ligating enzymes) were detected by Western blot. The levels of Ub, E1, E2 and E3 were up-regulated after induced by ox-LDL (grey level: 0.46 ± 0.08 vs 0.25 ± 0.09; 0.63 ± 0.09 vs 0.45 ± 0.09; 0.49 ± 0.07 vs 0.35 ± 0.06; 0.67 ± 0.05 vs 0.45 ± 0.05; ox-LDL 100 µg/ml, P < 0.05). The regulations peaked at 12 h and 24 h (12 h grey level: 0.58 ± 0.08 vs 0.25 ± 0.09; 0.72 ± 0.08 vs 0.45 ± 0.08; 0.65 ± 0.10 vs 0.35 ± 0.06; 0.65 ± 0.05 vs 0.43 ± 0.05, P < 0.05; 24h grey level: 0.72 ± 0.08 vs 0.25 ± 0.09; 0.79 ± 0.08 vs 0.45 ± 0.08; 0.72 ± 0.04 vs 0.35 ± 0.06; 0.65 ± 0.05 vs 0.43 ± 0.05, P < 0.05). The treatment of ox-LDL may activate the UPS to promote the degradation of HUVECs.
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