Abstract
The effect of osmolality of the incubation medium on the luminol-amplified chemiluminescence (CL) of human neutrophils stimulated by N-formyl-Met-Len-Phe (FMLP), phorbol-12-myristate-13-acetate (PMA), calcium ionophore A-23187, thermoaggregated IgG (aggIgG) and opsonized zymosan has been studied. It has been shown that increase of osmolality from 320 up to 420 mosM decreased the A-23187--and opsonized zymosan-induced CL responses by 90%. Under the same conditions PMA-, FMLP- and aggIgG-induced CL responses were decreased by 40-60%. The decrease of the osmolality of incubation medium resulted in 2-3 fold decrease of the A-23187-, PMA- and FMLP-induced CL and in 60-80% increase of opsonized zymosan- and aggIgG-induced CL. It is assumed that osmolality-mediated alteration of cell volume is an important regulatory step in the process of neutrophils' activation.
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