Changes of Soyasaponin Contents in Soybean Sprouts

Abstract

콩 재배 및 이용확대를 위한 기초자료를 얻기 위해 발아과정 중 기능성 성분인 soyasaponin의 함량 변화를 조사하였다. 1. Soyasaponin I과 soyasaponin II의 함량은 품종에 따라 차이가 있었으나 발아과정 중 떡잎부위에서 함량이 10배 이상 증가하였고, soyasaponin I은 배축에서도 종자 당 이 <TEX>$140{\sim}360{\mu}g$</TEX> 수준의 함량을 나타내었다. 2. Soyasaponin III 및 soyasaponin V의 함량은 발아를 통해 증가하는 경향이었고, soyasaponin V의 경우 배축에서 그 함량이 급격히 증가하는 것으로 나타났다. Soyasaponin I, II, III and V contents were investigated in seed, cotyledons and sprouts of soybean (Glycine max (L.) Merill) subjected to germination over five days. High-performance liquid chromatography coupled with tandem mass spectrometric (HPLC-MS/MS) method was used to evaluate the content of soyasaponins. Soyasaponins contents were different according to the varieties. Germination of soybeans dramatically increased soyasaponin contents in soybean sprouts in a time-dependent manner. Cotyledons had a higher contents of soyasaponins compared to dried seed (p<0.05). After five days of germination, Soyasaponin I and II increased 10 times higher after germination. Soyasaponin I and II are major metabolites in cotyledons and hypocotyls. Soyasaponin III and V were also detected in seed and increased depended on the germination stage. Soyasaponin V was at its highest levels in the hypocotyl, almost 7 times higher than the initial content in soybean seeds. Therefore, the germination of soybean sprouts significantly increased soyasaponin content.