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Abstract
Rat heart myoblasts (H9c2) were cloned and cultivated in Eagle's minimum essential medium supplemented with 10% FCS. Clones exhibiting high creatine kinase (CK) activity were investigated. Reduction of FCS to 2% for one week resulted in a differentiation of myoblasts towards myocytes. Surface morphology and ultrastructural changes of myoblasts and differentiated myocytes (transverse banding pattern) were investigated by means of electron and light microscopy.Following enzyme activities (nmoles/mg prot./hr) were measured in myoblasts and myocytes:
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