Changes of myeloid related protein-8/myeloid related protein-14 expressions in children with Kawasaki disease

Abstract

Objective To investigate the expression changes of myeloid-related protein-8(MRP-8) and myeloid-related protein-14(MRP-14) in children with Kawasaki disease(KD) and to obtain laboratory diagnostic serum markers and new targets for its drug therapy. Methods A total of 46 patients with KD(KD group) were enrolled from Jul.2009 to Dec.2010 and divided into the coronary artery dilatation(CAD) group(n=15) and the normal coronary artery group(n=31); Meanwhile, 25 febrile patients with acute respiratory tract infection but without disease in the circulatory, blood, immune systems formed the non-KD febrile group.Twenty healthy children from the out-patient department formed the healthy control group.Peripheral venous blood was collected in the acute and subacute stage of KD.Levels of MRP-8/MRP-14 were detected with enzyme-linked immunosorbnent assay(ELISA). Gene expressions of MRP-8, MRP-14 in leukocytes were analyzed by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR). Results The serum levels of MRP-8/MRP-14 along with mRNA expressions of MRP-8 and MRP-14 in the leukocytes in the out-patient acute and subacute stage of KD were significantly higher than those in the non-KD febrile group and the healthy control group(all P 0.05). The serum levels of MRP-8/MRP-14 along with mRNA expressions of MRP-8 and MRP-14 in leukocyte in actue stage of KD were significantly higher than those in subacute stage(all P<0.001). The serum levels of MRP-8/MRP-14 as well as mRNA expressions of MRP-8 and MRP-14 in the acute and the subacute stage of CAD group were significantly higher than those in the normal coronary artery group(P<0.05). Conclusions MRP-8/MRP-14 may probably play a role in the pathogenesis of KD and can be used as a diagnostic indicator for KD; MRP-8/MRP-14 may be involved in the formation of coronary artery lesion and can be used as an effective predictor for the coronary artery lesion. Key words: Kawasaki disease; Myeloid related protein-8; Myeloid related protein-14; Coronary arterial lesion