Changes of macro- and microelement composition of the liver in rats for artificial hypobiosis

Abstract

Surgeons throughout the world face numerous urgent questions as to alternative ways of anesthesia that don’t need to switch on apparatuses: “artificial heart”, “artificial lungs ventilation” at short-term surgical interventions. One of such ways can be just artificial hypobiosis. The main conditions for transferring to this stage are: hypoxia, hypercapnia, hypothermia. For using this method in surgical practice at short-term surgical interventions successfully in future, it is necessary to study a series of urgent questions, connected with its safety more detail. White usual male-rats with the mass 180–200 g, kept in standard vivarium conditions, were used in the study. The animals were divided in groups: control (intact animals) and experimental artificial hypobiosis state. The number of animals in each group n = 8. The experiments were conducted, according to requirements of the “European convention about protection of vertebral animals, used with experimental and other scientific purposes” (Strasburg, France, 1985), according to general ethic principles of experiments on animals, accepted by the First national congress of bioethics of Ukraine (2001). As a result of the conducted studies there was demonstrated the essential increase of Potassium, Sodium and Ferrum, at the same time the Calcium content decrease. The growth of Potassium and Sodium in the organism can be explained by the delay of liquid in the organism, because flowability of liquids in the organism decreases at artificially-created hypobiotic conditions