Changes of Interleukin-1β and Testosterone Concentration after Nonsteroidal Anti-Inflammatory Drug Treatment at Different Times Relative to Endotoxin Administration in an Equine Model

Janusz Danek,Ryszard Gołda,Wiesław Krumrych

doi:10.3409/fb65_2.107

Abstract

Changes of Interleukin-1β and Testosterone Concentration after Nonsteroidal Anti-Inflammatory Drug Treatment at Different Times Relative to Endotoxin Administration in an Equine Model

Highlights

The aim of the study was to determine the effect of a single dose of flunixin meglumine on serum interleukine-1â and serum and seminal plasma testosterone given at various times after the administration of endotoxin in stallions
Many of the deleterious effects of endotoxin are mediated by cytokines, among which most important are tumor necrosis factor-á (TNF-á), IL-6 and interleukin-1 which is referred to as IL-1á and IL-1â and proinflammatory substances (CALKINS et al 2002; CANNON et al 1990; CAVAILLON 1995; DEBOND et al 1996; CONTI et al 2004; MACKAY et al 1991; MACKAY & LESTER 1992; MORRIS et al 1990, 1992; DANEK 2002; SEETHANATHAN et al 1990)
In contrast to previous studies (DANEK 2006), the aim of this study was to determine the effect of a nonsteroidal anti-inflammatory drug – flunixin meglumine treatment on the changes of serum interleukin-1â and serum and seminal plasma testosterone concentration in endotoxemic stallions, especially in relation to the differential time injection of Flunixin meglumine (FM) after endotoxin administration

Summary

Introduction

The aim of the study was to determine the effect of a single dose of flunixin meglumine on serum interleukine-1â and serum and seminal plasma testosterone given at various times after the administration of endotoxin in stallions. In response to endotoxin infusion, the reaction of the stallions included fever (increased rectal temperature), increased heart rate, and leucopenia. Flunixin treatment prevented an endotoxin-induced increase in rectal temperature, heart rate, with no influence on the decrease of white blood cell numbers. FM (especially 30 minutes after LPS infusion) had a positive effect on the late changes of serum testosterone concentration after addition of endotoxin. Injection of FM (30 and 60 minutes after LPS administration) had a positive effect on the seminal plasma T level of the stallions.

Objectives

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Results

Conclusion