Changes of inositol phosphates during decapitation‐induced lateral bud break of Malus domestica

Abstract

An increase in phosphatidylcholine (PC), phosphatidyl‐ethanolamine (PE), phosphatidylglycerol (PG), and phosphatidylinositol (PI) occurred during bud break induced by decapitation. Inositol‐1‐phosphate [Ins(l)P1], inositol‐1,4‐bisphosphate [Ins(1,4)P2], and inositol‐1,4,5‐triphosphate [Ins(1,4,5)P3] were found in apple buds and increased progressively following decapitation. Ins(1)P1 and Ins(1,4)P2 peaked 48 h after decapitation and Ins(1,4,5)P3 peaked 72 h after decapitation during the metabolic transition when buds emerged from dormancy. Ins(1,4)P2 and Ins(1,4,5)P3 levels declined there after. The lateral buds on shoots with intact terminals and decapitated shoots treated with indole‐3‐acetic acid (IAA) in the terminals tip remained dormant and there were no significant changes in phospholipid and inositol phosphate contents.