Changes of Host Immunity Mediated by IFN-γ+ CD8+ T Cells in Children with Adenovirus Pneumonia in Different Severity of Illness.

Ruilin Zheng,Ning Han,Zhihui Ning,Haitian Chen,Mingqi Zhao,Bing Zhu,Yinghua Li,Danyang Chen,Jingyao Su,Misi Xiao

doi:10.3390/v13122384

Abstract

The host immunity of patients with adenovirus pneumonia in different severity of illness is unclear. This study compared the routine laboratory tests and the host immunity of human adenovirus (HAdV) patients with different severity of illness. A co-cultured cell model in vitro was established to verify the T cell response in vitro. Among 140 patients with confirmed HAdV of varying severity, the number of lymphocytes in the severe patients was significantly reduced to 1.91 × 109/L compared with the healthy control (3.92 × 109/L) and the mild patients (4.27 × 109/L). The levels of IL-6, IL-10, and IFN-γ in patients with adenovirus pneumonia were significantly elevated with the severity of the disease. Compared with the healthy control (20.82%) and the stable patients (33.96%), the percentage of CD8+ T cells that produced IFN-γ increased to 56.27% in the progressing patients. Adenovirus infection increased the percentage of CD8+ T and CD4+ T cells that produce IFN-γ in the co-culture system. The hyperfunction of IFN-γ+ CD8+ T cells might be related to the severity of adenovirus infection. The in vitro co-culture cell model could also provide a usable cellular model for subsequent experiments.

Highlights

The indicators of total protein, albumin, and alkaline phosphatase of adenovirus pneumonia patients were significantly decreased compared with the healthy control, as well as the indicators of γ-glutamyl transpeptidase and lactate dehydrogenase were significantly increased compared with the healthy control
Since the pathogenesis of adenovirus infection is still unclear, and there is a lack of specific treatment for adenovirus infection, the clinical treatment of patients with adenovirus infection is limited to symptomatic treatment
Cells that secrete IFN-γ, especially CD8+ T cells, which may be related to the pathogenesis of adenovirus infection

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. HAdV is a double-stranded DNA virus without an envelope. It belongs to the family of Adenoviridae. In 1953, HAdV was first isolated from human adenoid tissue culture by Rowe et al [1]. HAdV can be divided into seven species and more than

Methods

Results

Conclusion