Changes of glucose metabolism in mouse models of intracerebral hemorrhage: 18F-FDG microPET/CT study

Abstract

Objective To investigate the brain glucose metabolism with 18F-FDG microPET/CT in mouse models of intracerebral hemorrhage (ICH). Methods A total of 12 healthy adult male mice were randomly divided into sham operation group (group A, n=6) and ICH model group (group B, n=6) by simple random sampling method. The animal models were established by injecting collagenase Ⅳ into the caudate nucleus of mice. Thereafter (5.5±0.3) MBq of 18F-FDG was injected into caudal vein at 6 h, 24 h, 48 h and 3 d, 5 d, 8 d, 14 d, respectively, following anesthesia. 18F-FDG microPET/CT scans were acquired 30 min after the trace injection. SUV in the perihematomal brain tissue of ICH was measured and analyzed. Two-sample t test was used to compare SUV between groups. Results (1) Some mice had mild neurologic deficit after the sham operation in group A, while all mice had a marked neurologic deficit in group B, especially at 24 h after 18F-FDG injection. (2)After 6 h, FDG uptake in perihematomal brain tissue decreased(SUV=0.80±0.04), which significantly lower than that in the opposite side(SUV=1.10±0.04; t=2.69, P<0.05) and decreased to the minimum at 24 h(SUV=0.50±0.05). 18F-FDG uptake in perihematomal brain tissue began to increase at 3 d(SUV=1.20±0.05) and kept increasing during the 14 d observation. Compared with the group A, glucose metabolism in group B was significantly lower at each time point(t=37.67-86.60, all P<0.05). Conclusions 18F-FDG microPET/CT may dynamically reflect the changes of brain glucose metabolism in ICH mouse models. The FDG uptake in the center of ICH may disappear and the volume of hematoma with decreased uptake may shrink during the observation period. Key words: Cerebral hemorrhage; Tomography, emission-computed; Tomography, X-ray computed; Deoxyglucose; Mice