Changes of GFAP-immunoreactivity after partial food deprivation in the lateral septum of female and ovariectomized rats

Abstract

Event Abstract Back to Event Changes of GFAP-immunoreactivity after partial food deprivation in the lateral septum of female and ovariectomized rats Balázs Szőke1* and Katalin Halasy1 1 Szent Istvan University , Department of Anatomy and Histology, Hungary Neural elements in the lateral septum (LS) of rats react sensitively to food deprivation. It is still not known whether these changes include plastic rearrangements of synaptic connections. A reliable indicator of such changes is the detection of glial fibrillary acidic protein (GFAP), a specific marker of astroglia. In previous studies after partial food deprivation significant increase in the amount of GFAP-immunoreactive elements of the LS of male rats was observed. Our aim was to reveal density changes of GFAP-immunoreactive glial elements in intact and ovariectomized female rats after partial food deprivation. Female Wistar rats were grouped: intact and ovariectomized fed ad libitum (n=4-4), intact and ovariectomized food deprived (n=4-4). After one week animals were transcardially perfused with 4% paraformaldehyde then 60 m vibratome serial sections were cut from the LS in the coronal plane and incubated for the detection of GFAP at light microscopic level. Sections were digitally photographed and photos were evaluated with computer-assisted densitometry. Roughly even density of GFAP was measured along the rostrocaudal axis of the LS in control animals. As a result of partial food deprivation, a significant increase of GFAP-immunoreactive elements could be detected in the LS of both intact and ovariectomized female rats. Thus, the density of GFAP immunoreactivity seems to be independent of the amount of gonadal hormones, and the measured changes were due exclusively to food deprivation. Our previous and recent results prove that the LS react with marked neurochemical changes to partial food deprivation affecting both neural and glial elements. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Homeostatic regulatory mechanisms Citation: Szőke B and Halasy K (2009). Changes of GFAP-immunoreactivity after partial food deprivation in the lateral septum of female and ovariectomized rats. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.021 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Feb 2009; Published Online: 26 Feb 2009. * Correspondence: Balázs Szőke, Szent Istvan University, Department of Anatomy and Histology, Budapest, Hungary, aliscaszb@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Balázs Szőke Katalin Halasy Google Balázs Szőke Katalin Halasy Google Scholar Balázs Szőke Katalin Halasy PubMed Balázs Szőke Katalin Halasy Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.