Changes of gene expression profiles of infants inflammatory genes after Rota virus infection.

Abstract

Rotavirus is a medical important pediatric pathogen causes severe gastroenteritis in infants, leading to 450,000 deaths globally each year. RT-PCR-Array technique analysis is a powerful method to determine global profiles of gene expression in cells and tissues after viral infections. Using this analysis, the Ct values of 14 immune inflammatory genes were subjected to determine their gene expression rates compared with Ct values of control (non-infected persons). Five housekeeping genes were used as internal control genes. All mRNA transcripts of these genes were upregulated (from 2.2974 fold change for CCL3, CCR1, CCR3, CXCL2, IFN-a, IFNA-g, IL1B, IL1R and 21.1121 fold change for TNF-b) after infection with rotavirus. This results revealed that rotavirus infection increase the gene expression rate of 14 inflammatory genes and lead to high impact on key immune pathways in infected infants.