Abstract
The present study aimed to adapt a Long-run Real-time DNA Damage Quantification (LORD-Q) qPCR-based method for the analysis of the mitochondrial genome of Common carp (Cyprinus carpio L.) and detect the DNA damaging effect of T-2 (4.11 mg kg−1) and deoxynivalenol (5.96 mg kg−1) mycotoxins in a 3-week feeding period. One-year-old Common carp were treated in groups (control, T-2 and DON). The mycotoxins were sprayed over the complete pelleted feed, and samples were taken weekly. Following the adaptation of LORD-Q PCR method for the Common carp species, the number of lesions were calculated to determine the amount of DNA damage. In the first and second weeks, the T-2 and the DON treated groups differed significantly from each other; however these differences disappeared in the third week. There was a significant difference in the DNA lesion values between weeks 1 and 3 in the deoxynivalenol-contaminated groups. While in the T-2 treated groups, the DNA lesion values were significantly reduced on weeks 2 and 3 compared to week 1. The results suggested that the trichothecene mycotoxins have a relevant DNA damaging effect.
Highlights
Food and feed can be contaminated with different types and amounts of mycotoxins, and they have various damaging effects [1]
We describe the changes in the DNA damaging effect of T-2 and DON mycotoxins in a 3-week experimental period in Common carp (Cyprinus carpio L.)
It is important to be aware of the level of DNA damage and their evolution over time to understand the molecular effects, the mechanism of action and key elements of the process in many agents to connect the knowledge about oxidative stress, DNA structure changes, repair mechanisms and carcinogenesis
Summary
Food and feed can be contaminated with different types and amounts of mycotoxins, and they have various damaging effects [1]. PCR (Long-run Real-time DNA Damage Quantification PCR) is a highly efficent technique based on the principle that different DNA lesions halt the DNA replication and reduce the amount of PCR product [16] It is a fast and quantitative analytical method that has many advantages. We describe the changes in the DNA damaging effect of T-2 and DON mycotoxins in a 3-week experimental period in Common carp (Cyprinus carpio L.). For this purpose, a new LORD-Q PCR assay was optimized to detect and calculate the number of DNA lesions in mtDNA in vivo. DON and T-2 mycotoxins can trigger DNA lesions in a three-week-long period
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
