Changes of angiogenesis-related factors in type 2 diabetic patients with lower extremity angiopathy

Abstract

Objective To inveshgate the changes of angiogenesis-related factors,and evaluate the effects of angiogenesis on lower extremity angiopathy in patients with type 2 diabetes mellitus (T2DM).Methods Serum levels of CXCL16,basic fibroblast growth factor (bFGF),platelet-derived endothelial cell growth factor (PD-ECGF) and the number of endothelial progenitor cells (EPCs) were examined in 31 T2DM patients without lower extremity angiopathy( T2DM group),49 T2DM patients with lower extremity angiopathy( lower extremity angiopathy group),and 40 healthy controls (control group).Ankle brachial index (ABI),the inside diameter and blood velocity of arteria dorsalis pedis and arteria poplitea were measured with color Doppler ultrasonography.Results Serum levels of bFGF and PD-ECGF were significantly increased( P ＜ 0.05 ) and the number of EPCs were significantly decreased( P ＜ 0.001 ) in T2DM group compared with those in control group.The serum levels of CXCL16,bFGF and PD-ECGF were significantly increased ( P ＜ 0.05 ) and the number of EPCs were significantly decreased( P ＜ 0.01 ) in lower extremity angiopathy group compared with those in T2DM group and in control group.ABI,the inside diameter of arteria dorsalis pedis and arteria poplitea were significantly decreased (P ＜ 0.01 or P ＜ 0.05 ) in lower extremity angiopathy group compared with those in T2DM group and in control group.The blood velocity of arteria dorsalis pedis and arteria poplitea were significantly decreased(P ＜ 0.05) in lower extremity angiopathy group compared with those in control group.Conclusions There was angiogenic impairment in patients with T2DM.Increased angiogenesis-related factors and decreased number of EPCs may play important roles in lower extremity angiopathy of T2DM. Key words: Type 2 diabetes mellitus; Angiogenesis; CXCL16; Ankle brachial index