Changes of adenosine deaminase activity in serum and saliva around parturition in sows with and without postpartum dysgalactia syndrome

Marianne Kaiser,Magdalena Jacobson,José Joaquín Cerón,Stine Jacobsen,Jan Dahl,Damián Escribano,Poul Bækbo,Fernando Tecles,Pia Haubro Andersen

doi:10.1186/s12917-021-03067-6

Abstract

BackgroundPostpartum dysgalactia syndrome (PDS) is associated with a significantly higher activation of the inflammatory and stress response at parturition than in the healthy sow. Therefore, reliable and possibly non-invasive biomarkers for substantial increases of inflammation are searched to support the PDS diagnosis. This report studies the possible changes of the inflammatory marker enzyme adenosine deaminase (ADA) in serum and saliva of 38 PDS positive sows (PDS+) and 38 healthy sows (PDS-). Sampling was performed every 24 h from 60 h before to 36 h after parturition. Isoenzyme 1 (ADA1) and isoenzyme 2 (ADA2), as well as total ADA (tADA), were measured and their statistical association with several serum and saliva biomarkers of inflammation and stress was investigated.ResultsCompared to a baseline (60 to 36h prepartum), salivary activities of ADA1, ADA2 and tADA increased significantly over time in both PDS+ and PDS- sows, reaching their peaks after parturition. In serum from PDS- sows, no changes were observed over time in either ADA1, ADA2 or tADA. In PDS+ sows, serum ADA2 activity decreased temporarily after parturition followed by a significant increase compared to baseline. ADA1, ADA2 and tADA were all significantly associated with several inflammatory biomarkers and ADA1 in serum was associated with serum cortisol. Although serum activity was higher in PDS+ than in PDS- sows, the differences were not statistically significant. Further, no difference was noted between the groups in the analyses of saliva.ConclusionsSalivary ADA1 and ADA2 increased in all sows after parturition, potentially as a response to the postpartum inflammation. However, no difference in the activity of ADA1, ADA2 and tADA were found between PDS+ and PDS- sows indicating inability to diagnose PDS under the conditions described in this report.

Highlights

Postpartum dysgalactia syndrome (PDS) is associated with a significantly higher activation of the inflammatory and stress response at parturition than in the healthy sow
In the PDS+ group, adenosine deaminase 1 (ADA1), ADA2 and total ADA (tADA) were significantly increased in period E (0 to 12 h) as compared to the baseline values, and the highest value was obtained in period F (12 to 24 h)
No interaction was found between time and groups and, despite a strong trend for ADA2 (p = 0.0514), no significant difference was seen between the PDS+ and the PDS- sows

Summary

Introduction

Postpartum dysgalactia syndrome (PDS) is associated with a significantly higher activation of the inflammatory and stress response at parturition than in the healthy sow. This report studies the possible changes of the inflammatory marker enzyme adenosine deaminase (ADA) in serum and saliva of 38 PDS positive sows (PDS+) and 38 healthy sows (PDS-). Isoenzyme 1 (ADA1) and isoenzyme 2 (ADA2), as well as total ADA (tADA), were measured and their statistical association with several serum and saliva biomarkers of inflammation and stress was investigated. Increased ADA activity has been proposed as a biomarker of cell-mediated immunity, and its increase in serum can be considered a marker of inflammation [9]. Increased ADA activity has been found in saliva in humans with oral malignancies [20] and Sjögren’s syndrome [21]. ADA in saliva may be expected to be a useful marker of inflammation in pigs

Objectives

Methods

Results

Discussion

Conclusion