Abstract

Osmotic potentials (ψs) of squash hypocotyl sections increased when sections were aged in water. On the other hand, the ψs of sections aged in moist air or 0.15 M mannitol did not differ significantly from freshly excised tissues. The influence of the manner of ageing of hypocotyl tissues on urea uptake found in a previous study was possibly related to the effects of these treatments on ψs. For example, urea uptake was affected most strongly by the same ageing procedures that caused the greatest changes in ψs, especially incubation of hypocotyl sections in water. Because of greater losses of cellular solutes, the turgor pressures developed by tissues aged in water would have been lower than in other tissues when urea uptake was measured under standard hypotonic conditions. The present investigation supports the interpretation by a number of workers that turgor pressure may directly or indirectly modify permeability characteristics of plant cell membranes.

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