Changes in viability, respiratory activity and morphology of the marine Vibrio sp. strain S14 during starvation of individual nutrients and subsequent recovery

Abstract

Starvation for different individual nutrients revealed various morphotypes of Vibrio sp. strain S14. Carbon or multiple-nutrient starved cells formed ultramicrocells with low respiratory activity and high culturability. In contrast, cells starved for nitrogen or phosphorus formed filaments or swollen rods with large inclusion bodies of PHB. These cells exhibited a 3–4 log decrease in culturability, nevertheless many were actively respiring and direct viable counts equalled at least 80% of the original number of cells. After 2–3 days of prolonged incubation, microcolonies appeared at approximately the same number of cells as at the onset of starvation. A nutrient-induced increase in respiratory activity, after 120 h of starvation, was instantaneous for cells starved for carbon or multiple-nutrients, but cells depleted of nitrogen or phosphorus exhibited a lag period of at least 3 h.