Changes in urinary level and configuration ratio of d-lactic acid in patients with short bowel syndrome

Abstract

The present study showed that the d-lactic acid configuration ratio in the urine rose earlier than that in blood or the urinary or blood d-lactic acid levels upon disease onset, and that the d-lactic acid measurement in urine is more sensitive and useful than that in blood. As this result, a prediction of a d-lactic acidosis may be possible. To simplify the procedure for detecting d-lactic acid, we first showed a correlation between the d-lactic acid configuration ratio in urine and blood, indicating urine could be used. To separate the optical isomers of lactic acid, we simplified our previous procedure. For chiral recognition, we chose O-acetyl-(−)-menthylation and analyzed the samples under GC/MS by capillary gas chromatography on a DB-5MS column. This procedure is less sensitive than the former method, but it is faster and simpler, requiring only one derivatization step. This method may be useful for predicting d-lactic acidosis in patients with short bowel syndrome.