Abstract
Oocyte maturation (meiosis reinitiation) in the catfish ( Clarias batrachus) is induced by maturation-inducing steroid (MIS), 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DP). The results of the present study demonstrate the possible involvement of cAMP in oocyte maturation in vivo or induced by 17α,20β-DP in vitro. Measurement of total cAMP levels at different morphological stages of oocytes obtained under in vivo conditions showed a significant reduction between the values at the centrally located germinal vesicle (C-GV), subperipheral (SP-GV), peripheral (P-GV), and germinal vesicle breakdown (GVBD) stage. In the time course study, MIS-treated oocytes showed a significant reduction in the total cAMP level at 2 hr and maintained it up to the duration of the study (24 hr). These results suggest that a decrease in the cAMP level within the follicle triggers oocyte maturation in this species. The in vitro effects of the adenylate cyclase activator, forskolin, and the phosphodiesterase inhibitor, theophylline, on 17α,20β-DP-induced GVBD was also investigated. Both substances inhibited 17α,20β-DP-induced GVBD, suggesting indirectly the inhibitory role of cAMP in catfish oocyte maturation.
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