Changes in Thiols Indicators in the Lens of the Eye of White Rats under the Exposure of Radiation during Operation of a Cellular Mobile Phone

Abstract

The results of previous work carried out at the Laboratory of Biophysics of Cellular Metabolism of the Institute of Physiology named after A.I. Karaev, which concerned the study of the effect of EMR on LPO processes and the antioxidant system in visual structures, pointed to the oxidative nature of the implementation of EMR exposure. Since thiol compounds in living tissues primarily interact with peroxide products, it was assumed that the effect of EMR should be reflected in the thiol content of the lens. For this purpose, a group of white outbred rats (40 species, half of which were identified as the control group) were studied, which were daily irradiated with EMR from a working cell phone. On the 7th, 14th, 21st and 28th day of the experiment, 5 animals from the experimental group and 5 animals from the control group were slaughtered. The lens of the eye was removed from the animals, in which the level of thiols was determined according to the modified Lindsey-Sedlak method based on the Ellman method. The results of the study showed that common and readily available (cytoplasmic) thiols in the nucleus of the lens of the eyes of rats irradiated with mobile phone EMR, in general, have similar dynamics of quantitative changes depending on the duration of exposure. Hidden thiols behave similarly. In the lens cortex, the nature of changes in thiols is not so pronounced, but generally similar to the results in the nucleus. The authors believe that these results may indicate the antioxidant properties of thiols in LPO reactions.