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Abstract
Changes in the Relative Abundance of miR-205, miR-26a-5p, let-7b and their Target Genes in Vitrified Bovine Embryos after Phenazine Ethosulfate Supplementation
Highlights
Many genetic companies that are concerned with artificial insemination field have started operating in embryo market, which has resulted in an increasing demand in in-vitro (IVP) embryos
It was found that miRNAs regulate many genes in stage specific manner, during different stages of embryonic developmental (Kropp and Khatib,2015; Salilew-Wondim et al.,2020).,it was shown that embryos are well equipped with a huge amount of maternal miRNAs that inherited by embryos until activation of early embryonic genome (Tesfaye et al, 2018)
The current work aimed to investigate the influence of phenazineethosulfate (PES) supplementation in culture media on the selected miRNAs and their target genes (OCT4, DNA methyltransferase (DNMT), CASP3, activating transcription factor 6 (ATF6), ATP5ME, and ELOVL5), during bovine embryo production
Summary
Many genetic companies that are concerned with artificial insemination field have started operating in embryo market, which has resulted in an increasing demand in in-vitro (IVP) embryos. This strategy resulted in a profound change in global cattle genetic market (Marsico et al, 2019). The great efforts have been done by researchers to improve IVP during the last years, the efficiency remains low (Sudano et al, 2019). The IVP embryos are less resistant to cryopreservation and revealed lower survivability compared with in vivo produced, because of increased lipid droplets (Sanches et al, 2017).
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