Changes in the proximate and elemental composition of Alitropus typus (Crustacea: Flabellifera: Aegidae) exposed to lethal dose of bacterial consortium.

Abstract

Alitropus typus is a crustacean parasite, which is increasingly becoming a menace to aquafarmers. In our previous study, a novel microbial consortium comprising of three exoskeleton degrading bacterial strains (Stenotrophomonas maltophilia, Bacillus altitudinis and Klebsiella pneumoniae) had shown promising results as a biocontrol agent for A. typus. The present investigation reports the changes in proximate and elemental composition associated with the application of microbial consortium on the isopod A. typus. Proximate analysis showed an increased level of protein, lipid, and moisture in treated isopod at 48h compared with untreated isopod. However, ash and chitin concentrations were lower in treated isopod. The elements in the mid-tergite of untreated isopod was compared with the treated isopod at 48h using scanning electron microscopy and energy dispersive x-ray spectroscopy (SEM-EDAX). The following elements were analyzed in the mid-tergite segment of untreated isopod: C, O, Na, Mg, Al, Si, P, S, Cl, K, Ca and Fe. The results showed that the concentration of calcium had decreased significantly in the treated isopod at 48h (4.28 ± 0.11%) when compared to the untreated isopod (10.01 ± 0.32%), indicating that the bound form of calcium carbonate in the exoskeleton had been precipitated by microbial action. The concentration of carbon and phosphorous was higher in the treated isopods at 48h compared to the untreated ones. The data suggests that treatment with microbial consortium is not only an effective but also an environmentally safe alternative for the control of A. typus.