Abstract
Kidney tissue is particularly susceptible to reactive oxygen species attack which leads to development of cancer. During oxidative stress, membrane lipids and proteins are major targets of re-active oxygen species (ROS). This work is focused on changes of phospholipids, proteins content and electric charge that occur in cell membranes of kidney cancer of pT3 stage, grade G3 and with metastasis. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were determined by high-performance liquid chromatography. Electrophoresis was used to determine the surface charge density of the human kidney cell membrane. It was shown that the process of cancer transformation was accompanied by an increase phospholipid levels and altered the level of integral proteins as determined by decrease phenylalanine, tyrosine, cysteine and arginine. Moreover, the process of cancer transformation significantly enhanced changes in the surface charge density of the human kidney cell membrane. Cell membrane structure and function are modified by neoplasm lesion.
Highlights
Cell membrane is an integral part of an alive cell and it plays an essential role in life processes
Our previous experiments demonstrated that the cell membrane and the function are modified during cancer transformation. It is reflected by changes in the amount of phospholipids, proteins and free fatty acids in human colorectal cancer cell membrane [2] [5]-[7]
We worked out an average of 8 patients because the changes observed in patients 1 - 8 proved to be similar
Summary
Cell membrane is an integral part of an alive cell and it plays an essential role in life processes. How to cite this paper: Szachowicz-Petelska, B., Dobrzyńska, I., Figaszewski, Z.A. and Kudelski, J. (2014) Changes in the Physico-Chemical Properties of Human Kidney Cell Membranes during the Cancer Transformation. Portant properties of a biological membrane are its electric charge and its potential drop between the membrane and surrounding solution. Electric properties of the membrane are determined by acid-base and complex formation equilibria of membrane and solution components [1]. Most membrane components—phospholipids and proteins—are involved in those equilibria
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