CHANGES IN THE PATTERNS OF BIOSYNTHESIS and COMPOSITION OF AMINO ACIDS IN A MARINE PHYTOPLANKTER EXPOSED TO ULTRAVIOLET‐B RADIATION: NITROGEN LIMITATION IMPLICATED

Abstract

Abstract— The impact of ultraviolet‐B radiation (UVBR) on the biosynthesis and the composition of intracellular dissolved free and combined amino acids was examined in nitrate‐replete and nitrate‐deficient cultures of Tetraselmis sp. Several similarities were observed in the response of Tetraselmis sp. to nitrogen deficiency and exposure to UVBR, in support of a view that UVBR affects amino acid synthesis in phytoplankton via its inhibitory effects on nitrogen assimilation into the cells. At levels that still permitted the uptake of carbon into the cells, both nitrogen deficiency and UVBR exposure resulted in a reduction in the overall rates of carbon incorporated into amino acids, an increase in the absolute concentrations of amino acids within the intracellular dissolved free amino acid (INDFAA) pool and a decrease in the total cellular amino acid (TCAA) pool. An examination of the patterns of carbon assimilation into individual amino acids in cells exposed to UVBR revealed similarities with the patterns in cells subject to nitrogen deficiency. The most conspicuous changes from the controls included an increased incorporation of 13C into glutamic' acid (glutamic acid + glutamine) and aspartic acid and a marked reduction into alanine and valine. Changes in the concentrations of amino acids within the INDFAA and TCAA pools were also similar in nitrate‐deficient and UVBR‐exposed cells and resembled the carbon assimilation patterns. These results strongly suggest that UVBR‐induced changes in the biosynthesis and composition of amino acids are probably via its suppression of nitrogen assimilation into the cells.