Changes in the levels of glucose 1,6-diphosphate and atp and in the activities of phosphofructokinase and phosphoglucomutase induced by local anesthetics in the isolated rat diaphragm muscle

Abstract

1. 1. Local anesthetics, lidocaine and procaine, which were reported to cause muscle damage, induced a marked decrease in the level of glucose-l,6-diphosphate (Glc-l.6-P 2) in the isolated rat diaphragm muscle. 2. 2. Concomitant to the decrease in Glc-l.6-P 2, the powerful activator of phosphofructokinase and phosphoglucomutase, the activities of these enzymes were significantly reduced. 3. 3. Both local anesthetics also exerted a decrease in the concentration of muscle ATP, the effect of lidocaine being more pronounced than that of procaine. 4. 4. All these changes induced by the local anesthetics closely resembled dystrophic muscle, suggesting a common mechanism associated with the ultrastructural damage of muscle in both conditions.