Abstract
The aim of the present study was to investigate inline lactate dehydrogenase (LDH) changes in clinically healthy dairy cows according to the number and stage of lactations, milk yield, and the reproduction status The LDH activity (μmol/min per litre) was measured using the dry-stick technology. A total of 378 cows were selected and classified according to their reproductive status into the following groups: fresh (1–44 days after calving); open (45–65 days after calving); inseminated (1–35 days after insemination); pregnant (35–60 days after insemination). According to their milk productivity, the cows were classified into the following groups: <15 kg/d, 15–25 kg/d, >25–35 kg/d, and >35 kg/d. They were milked with a DeLaval milking robot in combination with a Herd Navigator analyser. The results showed that the inline LDH concentration had a tendency to increase along with the increase in the number of lactation periods (P < 0.05). The highest level of LDH was observed in fresh cows 5–10 days in milk (DIM), and the highest LDH concentration was found in the milk of fresh cows. A positive statistically reliable relationship was found between the milk yield and LDH concentration (P < 0.05); LDH and milk somatic cell counts (SCC) were positively related in all groups of cows, although LDH concentration and SCC were the highest correlated variables in inseminated cows (P < 0.05). The present study shows that measuring LDH activity in milk is both easy and cost effective with high sensitivity and specificity, having a great potential as a diagnostic tool for detection of subclinical mastitis.
Highlights
With inline monitoring, level shifts can be detected in real time as new observations are made
The present study shows that measuring lactate dehydrogenase (LDH) activity in milk is both easy and cost effective with high sensitivity and specificity, having a great potential as a diagnostic tool for detection of subclinical mastitis
95% PI- confidence interval is a cytoplasmic enzyme involved in carbohydrate metabolism that gets released into milk from ruptured mammary epithelial cells, phagocytes and from serum resulting in a change in the physical and chemical properties of milk during mastitis (Singh et al 2016)
Summary
Level shifts can be detected in real time as new observations are made. Previous studies have found a strong positive correlation between LDH and SCC (Zank and Schlatterer 1998; Hiss et al 2007), and LDH is generally accepted as a useful mastitis indicator. The challenge of distinguishing between cows with latent infections and healthy cows based on LDH measurements has been described (Hiss et al 2007). According to Nyman et al (2014), further studies are needed to investigate whether the diagnostic properties of LDH will improve with adjustment according to their relationship with other different cow factors when used as a diagnostic tool for finding cows with mastitis. The aim of the present study was to investigate inline LDH dynamic changes according to different cow factors –the number and stage of lactations, milk yield, and the reproduction status in clinically healthy dairy cows
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