Changes in the hemolymph lipophorin and very high density lipoprotein levels during the fifth nymphal and adult stages of Triatoma infestans

Abstract

The occurrence of lipophorin (HDLp) and a very high density lipoprotein (VHDL) throughout the last nymphal and adult stages of male T. infestans was followed by quantitative immunodiffusion using specific antisera derived from the purified lipoproteins of adult male insects. Significant changes on the concentration of these hemolymph lipoproteins (Lps) occurred during the mentioned developmental stages. These changes include the following. A great accumulation of hemolymph proteins and Lps, particularly VHDL, during the nymphal stage. This increase reached a peak just before the last molt, where the concentration of VHDL and HDLp were 74 and 42 mg/ml hemolymph, respectively. A sharp decline in the Lps concentration just after the molt and during the first 2 weeks of adult life. These changes imply a six-fold decrease on the VHDL and a two-fold decrease in the HDLp concentrations. Another increase in the protein concentration that begins around the third week of the adult stage affecting both Lps, but mainly the HDLp. From its hexameric structure, amino acid composition and high concentration in nymphal stages, the VHDL of T. infestans could be considered a storage protein. The fact that VHDL persists in a considerable concentration in the hemolymph of adult insects differenciates this VHDL from this protein. The distribution of 14C free fatty acid (FFA) among the hemolymph proteins of T. infestans shows that the FFA are associated only with VHDL and HDLp. The developmental changes of the Lps pattern are accompanied by changes in the relative distribution of FFA between these Lps. The VHDL is the principal carrier of FFA during the fifth-nymph stage while HDLp is the main protein and FFA-carrier in adult life.