Changes in the enterobacterial flora and the hydrogen gas-producing ability of the enterobacterial flora in a mouse model of acute lung injury

Abstract

Aims: The aims of the present study were to evaluate the changes in the enterobacterial flora and evaluate the hydrogen gas-producing ability of the enterobacterial flora in a mouse model of severe acute lung injury (ALI mouse model). Methods: The ALI mouse model was prepared by administering lipopolysaccharide (LPS) after activation of natural killer cells with α-galactosylceramide. The exhaled hydrogen concentrations of the mice were measured. The oxidative stress of the lung was evaluated by measuring the myeloperoxidase (MPO) activity. At the same time, feces were collected from the cecum of the mice and the hydrogen gas-producing ability was measured. Metagenomic analysis was also carried out to evaluate the changes in the enterobacterial flora. Results: The MPO activity in the lung of the ALI mouse model was significantly increased as compared to that of the control (n=25; P Conclusion: The ALI mouse model showed an increased hydrogen gas-producing ability of their enterobacterial flora, along with a change in the composition of the enterobacterial flora, which may protect organs against severe inflammation.