Abstract
When Hiproly barley callus was placed on an auxin-free Murashige and Skoog’s medium, adventitious roots were formed. The contents of S-adenosylmethionine (SAM) and 1-methylhistidine increased gradually in the callus throughout the root differentiation, along with increases in the levels of their precursors, methionine, histidine, and adenosine 5′-triphosphate. In the callus after auxin withdrawal, the addition of methionine resulted in rapid increases in the content of SAM and the incorporation of [methyl-14C]methionine into SAM. The accumulation of SAM was closely related to the elevation in methionine level in the callus. 1-Methylhistidine also increased greatly in the callus treated with methionine, but the addition of histidine had little effect on the change in 1-methylhistidine in the callus.
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