Changes in the Cell Wall Proteome of Leaves in Response to High Temperature Stress in Brachypodium distachyon.

Artur Pinski,Urszula Jankowska,Elisabeth Jamet,Robert Hasterok,Bozena Skupien-Rabian,Alexander Betekhtin

doi:10.3390/ijms22136750

Abstract

High temperature stress leads to complex changes to plant functionality, which affects, i.a., the cell wall structure and the cell wall protein composition. In this study, the qualitative and quantitative changes in the cell wall proteome of Brachypodium distachyon leaves in response to high (40 °C) temperature stress were characterised. Using a proteomic analysis, 1533 non-redundant proteins were identified from which 338 cell wall proteins were distinguished. At a high temperature, we identified 46 differentially abundant proteins, and of these, 4 were over-accumulated and 42 were under-accumulated. The most significant changes were observed in the proteins acting on the cell wall polysaccharides, specifically, 2 over- and 12 under-accumulated proteins. Based on the qualitative analysis, one cell wall protein was identified that was uniquely present at 40 °C but was absent in the control and 24 proteins that were present in the control but were absent at 40 °C. Overall, the changes in the cell wall proteome at 40 °C suggest a lower protease activity, lignification and an expansion of the cell wall. These results offer a new insight into the changes in the cell wall proteome in response to high temperature.

Highlights

Published: 23 June 2021In recent years, plant productivity has been increasingly threatened by climate change, primarily extreme climate events, altered rainfall patterns and increasing global mean land and ocean surface temperatures [1]
The 1D-electrophoretic patterns were slightly different between the control (21 ◦ C) and plants subjected to 40 ◦ C treatment (Figure A1)
We focused on the changes in the cell wall proteome in response to high

Summary

Introduction

Published: 23 June 2021In recent years, plant productivity has been increasingly threatened by climate change, primarily extreme climate events, altered rainfall patterns and increasing global mean land and ocean surface temperatures [1]. Multimethod analyses predict that each Celsius degree increase in the global mean temperature will negatively impact the yields of four major crops: maize, wheat, rice and soybean, which will result in food scarcity. In order to cope with the negative impact of climate changes and need to increase food production, there is an urgency to develop new climate-smart crops that fit into the climate-smart agriculture approach [3,4,5]. The development of such crops can be done using genetic engineering and breeding as well by exploiting epigenetic variations [6,7]. In order to facilitate the development of climate-smart crops, Brachypodium distachyon was proposed

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