Abstract
The synthesis and function of dermatan sulfate in peritoneal polymorphonuclear (PMN) leukocytes were studied. The peritoneal PMN leukocytes were obtained at 4,8, and 16 h after guinea pigs were injected intraperitoneally with caseinate solution, and were incubated with [35S] sulfate or [3H] glucosamine on plastic. The total glycosaminoglycan synthesis and the proportion of dermatan sulfate to total glycosaminoglycans linearly increased with time. In order to clarify the function of the increased dermatan sulfate, peritoneal PMN leukocytes were cultured with [35S] sulfate on plastic or collagen gel. The total glycosaminoglycan synthesis on the collagen gel culture increased 1.5 times compared with that on the plastic culture, and especially, dermatan sulfate synthesis increased twofold. In addition, 65% of dermatan sulfate on the collagen gel culture was found in the cell and the collagenase-soluble fractions. These results indicate that proteodermatan sulfate synthesized by PMN leukocytes interacts with collagen in vitro, and suggest that PMN leukocytes, which migrated to the inflammatory locus, lastly adhere to the injured tissue through the interaction of proteodermatan sulfate synthesized by themselves with collagen fibers exposed in the inflammatory locus.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
