Changes in the biochemical parameters of blood and the morphological structure of the pancreas in rats with acute pancreatitis and their correction using corvitin

Abstract

Acute pancreatitis (AP) is an inflammation of the pancreas characterized by a severe course and a high mortality rate. The pathogenesis of AP is still not fully understood, so there is currently a lack of treatment. Corvitin is a water-soluble form of quercetin that retains all the properties of quercetin and has powerful antioxidant, anti-inflammatory and anti-apoptotic properties. The aim of our study was to evaluate the effect of corvitin on biochemical blood parameters and morphological features of the pancreas in rats with AP caused by intraperitoneal administration of L-arginine (200 mg/100 g). This model of AP is non-invasive, highly reproducible and causes selective, dose-dependent necrosis of acinar cells and is ideal both for studying the pathomechanisms of AP and for observing and influencing changes in the course of the disease. The legality of using this model is confirmed by the morphostructural changes in the pancreas that are characteristic of AP. In rats with AP, an increase in the blood concentration of α-amylase (twice), alanine aminotransferase (ALT) (three times), aspartate aminotransferase (AST) (one and a half times) compared to control values was observed already on the first day of the pathology development. On the second day of AP, the level of glucose and urea in the blood of rats increased by 34 and 22%, respectively, while the creatinine content did not change. Under the influence of corvitin (50 mg/kg), the levels of α-amylase, ALT and glucose decreased already after a day, while the content of AST and urea increased and remained so until the 8th day of observation. The most positive dynamics of morphological changes in the pancreas of rats was observed when corvitin was used on the first day of AP induction.