Abstract

This study aimed to evaluate changes in the antioxidant properties of rice bran protein isolate (RBPI) upon simulated gastrointestinal digestion. A two-stage in vitro digestion system (pepsin treatment followed by pancreatin treatment) was used to generate GI-digested RBPI. Antioxidant activities were evaluated using various oxidative systems: measurement of oxygen radical antioxidant capacity (ORAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS+) assay, and reducing power assay. After gastrointestinal digestion, the protein solubility and free phenolic compounds increased from 24.6% to 94.5% and from 2.1 to 17.6 mg/g, respectively. The ORAC value of the GI-digested RBPI was significantly higher (p < 0.05) than that of the intact RBPI (473.5 vs. 44.2 μmol Trolox equivalents/g); furthermore, the GI-digested RBPI also showed significantly higher DPPH & ABTS radical-scavenging activities and reducing power than intact RBPI (p < 0.05). This study provides novel quantitative insights into the antioxidant properties of GI-digested RBPI in various oxidation systems and indicates that simulated gastrointestinal digestion is potentially applicable for producing bioactive components with higher antioxidant potentials than those of RBPI.

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